8YV6

Crystal structure of LC3B in complex with Influenza A virus M2 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-07-20
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	35.681, 38.462, 93.774
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.887 - 1.750
R-factor	0.2118
Rwork	0.210
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.183
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.890	1.840
High resolution limit [Å]	1.750	1.750
Number of reflections	13649	1935
<I/σ(I)>	27.9
Completeness [%]	100.0
Redundancy	12.8
CC(1/2)	1.000	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30% Jeffamine, 0.1M HEPES, pH 7.0