8YV5

The crystal structure of G. acetivorans RNA kinase Ark1 in complex with ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-24
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9792
Spacegroup name	I 21 3
Unit cell lengths	146.920, 146.920, 146.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.940 - 1.980
R-factor	0.1801
Rwork	0.178
R-free	0.21630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.266
Data reduction software	HKL-3000 (7.21)
Data scaling software	HKL-3000 (7.21)
Phasing software	PHASER (2.7.0)
Refinement software	PHENIX ((1.19.1_4122: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.460	2.030
High resolution limit [Å]	1.980	1.980
Number of reflections	36696	36696
<I/σ(I)>	19.7
Completeness [%]	100.0
Redundancy	35
CC(1/2)	0.999	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1M Sodium acetate trihydrate pH4.6 2.0M Sodium chloride