8YUE
Crystal structure of the kinesin-14 motor protein from Drosophila melanogaster
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 164.506, 67.617, 94.196 |
| Unit cell angles | 90.00, 96.99, 90.00 |
Refinement procedure
| Resolution | 46.750 - 3.150 |
| R-factor | 0.2532 |
| Rwork | 0.251 |
| R-free | 0.29470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.574 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20_4459: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.500 | 3.320 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rpim | 0.138 | 1.000 |
| Number of reflections | 17466 | 2747 |
| <I/σ(I)> | 6.9 | |
| Completeness [%] | 96.1 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296 | 0.5M NaCl, 50mM NaPi, pH6.8, 7mM DTT, 13% PEG 8000 |
