8YTU

Mipa-PETase from Micromonospora pattaloongensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2020-11-12
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	49.067, 103.411, 131.875
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.820 - 1.340
R-factor	0.21202
Rwork	0.211
R-free	0.23437
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.446
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.820	1.410
High resolution limit [Å]	1.340	1.340
Number of reflections	136706	115359
<I/σ(I)>	0.364
Completeness [%]	96.1
Redundancy	2.6
CC(1/2)	0.990	0.200

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10.5	293	PEG 8000, CAPS