8YTN

Single-chain Fv antibody of E11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.8
Spacegroup name	I 2 2 2
Unit cell lengths	33.620, 85.640, 178.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.800 - 1.720
R-factor	0.1863
Rwork	0.185
R-free	0.21270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6k4z
RMSD bond length	0.006
RMSD bond angle	0.933
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.850
High resolution limit [Å]	1.720	1.720
Rmerge	0.258	5.351
Number of reflections	28037	4305
<I/σ(I)>	10.5	0.9
Completeness [%]	100.0
Redundancy	19.4
CC(1/2)		0.591

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	4.0 M sodium formate