8YTI

Crystal Structure of Nucleosome-H1x Linker Histone Assembly (sticky-169a DNA fragment)

Replaces:  7XX7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	98
Detector technology	PIXEL
Collection date	2015-09-19
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	104.852, 102.111, 216.179
Unit cell angles	90.00, 96.34, 90.00

Refinement procedure

Resolution	92.220 - 2.700
Rwork	0.210
R-free	0.26690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.229
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.220	2.850
High resolution limit [Å]	2.700	2.700
Number of reflections	124272	17749
<I/σ(I)>	9.8
Completeness [%]	99.6
Redundancy	3.4
CC(1/2)	0.998	0.521

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	40-45 mM CaCl2, 25 mM KCl, 10 mM Na-acetate (pH 4.5)