8YTG
Crystal structures of human IRF2BP2 RING domain in complex with IRF2 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 26.733, 39.868, 36.247 |
| Unit cell angles | 90.00, 108.21, 90.00 |
Refinement procedure
| Resolution | 34.430 - 1.450 |
| R-factor | 0.1661 |
| Rwork | 0.165 |
| R-free | 0.18870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.873 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.870 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.163 | 0.406 |
| Number of reflections | 12812 | 1270 |
| <I/σ(I)> | 10.3 | 1.8 |
| Completeness [%] | 98.9 | |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.5 M ammonium sulfate and 0.1 M sodium acetate (pH 4.6) |
