8YTF

Crystal structures of human IRF2BP2 RING domain in complex with VGLL4 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9752
Spacegroup name	P 21 21 21
Unit cell lengths	33.372, 43.209, 55.761
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.430 - 1.590
R-factor	0.1536
Rwork	0.153
R-free	0.17270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.064
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.620
High resolution limit [Å]	1.590	4.320	1.590
Rmerge	0.085	0.051	0.327
Rmeas	0.088	0.053	0.341
Rpim	0.024	0.015	0.094
Total number of observations	141058
Number of reflections	11169	637	483
<I/σ(I)>	5.2
Completeness [%]	98.9	98.8	92.9
Redundancy	12.6	11.3	12.3
CC(1/2)	0.998	0.999	0.975

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% 2-proponal, 0.1 M MES (pH 6.0) and 20% (w/v) PEG MME 2000