8YT7

Crystal structure of the MAM domain of Spodoptera frugiperda Scavenger Receptor-C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9798
Spacegroup name	P 32 2 1
Unit cell lengths	57.020, 57.020, 159.934
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.020 - 2.250
R-factor	0.2485
Rwork	0.247
R-free	0.26880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.323
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.335
High resolution limit [Å]	2.250	2.250
Number of reflections	14876	1443
<I/σ(I)>	38.67
Completeness [%]	99.6
Redundancy	9.9
CC(1/2)	0.997	0.551

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2M magnesium chloride,20% poly ethylene glycol 3350, pH5.9