8YSW
phosphinothricin dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.665, 129.068, 219.138 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.360 - 2.610 |
| R-factor | 0.1956 |
| Rwork | 0.192 |
| R-free | 0.25710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.952 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASES |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.360 | 2.703 |
| High resolution limit [Å] | 2.610 | 2.610 |
| Rmerge | 0.187 | 1.379 |
| Rmeas | 0.195 | 1.435 |
| Rpim | 0.054 | 0.395 |
| Number of reflections | 80621 | 7955 |
| <I/σ(I)> | 12.55 | 2.14 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.1 | 13.1 |
| CC(1/2) | 0.997 | 0.752 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M Bis Tris Propane, pH 6.5, 0.2 M Sodium malonate dibasic monohydrate, 20% w/v PEG 3350, 10 % v/v Ethylene glycol |
