8YSV
Crystal structure of beta - glucosidase 6PG from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 291.15 |
| Detector technology | PIXEL |
| Collection date | 2023-10-02 |
| Detector | DECTRIS PILATUS 12M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.110, 79.000, 58.080 |
| Unit cell angles | 90.00, 106.03, 90.00 |
Refinement procedure
| Resolution | 30.278 - 1.790 |
| R-factor | 0.1895 |
| Rwork | 0.187 |
| R-free | 0.22950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f66 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.837 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.16_3549: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.220 | 1.840 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.159 | 1.781 |
| Number of reflections | 40049 | 2937 |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 99.7 | |
| Redundancy | 6.68 | |
| CC(1/2) | 0.529 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291.15 | Ammonium sulfate,HEPES pH7.0 |
