8YSH

MERS-CoV RBD in complex with nanobody Nb14

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-28
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 41 21 2
Unit cell lengths	58.488, 58.488, 351.625
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.110 - 1.990
R-factor	0.2293
Rwork	0.229
R-free	0.23920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.092
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	87.910	2.090
High resolution limit [Å]	1.990	1.990
Number of reflections	43747	4239
<I/σ(I)>	17.5
Completeness [%]	99.9
Redundancy	24.9
CC(1/2)	0.999	0.937

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	10% w/v PEG 6000, 8% Ethylene glycol, 0.1 M Citric acid pH 3.5