8YSG

Crystal structure of the Deinococcus wulumuqiensis CD-NTase DwCdnB in complex with GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-11
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.99984
Spacegroup name	P 1 21 1
Unit cell lengths	35.033, 100.404, 46.398
Unit cell angles	90.00, 101.62, 90.00

Refinement procedure

Resolution	22.734 - 2.171
Rwork	0.242
R-free	0.27180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.393
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.250
High resolution limit [Å]	2.170	2.170
Rmerge	0.028
Number of reflections	16522	1572
<I/σ(I)>	47.211
Completeness [%]	99.3
Redundancy	4.2
CC(1/2)		0.979

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2 M sodium formate, 20 % w/v PEG 3350