8YRT
Crystal structure of D-amino acid transaminase from Haliscomenobacter hydrossis in the holo form obtained at pH 7.0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-05-30 |
Detector | RIGAKU HyPix-6000HE |
Wavelength(s) | 1.54 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 86.043, 71.902, 52.015 |
Unit cell angles | 90.00, 100.14, 90.00 |
Refinement procedure
Resolution | 21.960 - 2.000 |
R-factor | 0.14716 |
Rwork | 0.145 |
R-free | 0.19038 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 2.285 |
Data reduction software | CrysalisPro |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.960 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.075 | 0.391 |
Rmeas | 0.081 | 0.425 |
Rpim | 0.032 | 0.164 |
Total number of observations | 139425 | 9847 |
Number of reflections | 21090 | 1484 |
<I/σ(I)> | 23.7 | 4.9 |
Completeness [%] | 99.7 | |
Redundancy | 6.6 | 6.6 |
CC(1/2) | 0.998 | 0.926 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 288 | 45% v/v Tacsimate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0 |