8YQ7

Acinetobacter baumannii membrane-bound lytic murein transglycosylase G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-06-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97959
Spacegroup name	P 1 21 1
Unit cell lengths	70.860, 162.370, 79.110
Unit cell angles	90.00, 108.96, 90.00

Refinement procedure

Resolution	29.470 - 2.670
R-factor	0.2402
Rwork	0.238
R-free	0.27290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.961
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.470	2.765
High resolution limit [Å]	2.670	2.670
Number of reflections	47837	4749
<I/σ(I)>	12.28
Completeness [%]	99.6
Redundancy	7.1
CC(1/2)	0.998	0.712

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	17%(w/v) PEG 10000, 0.1M BisTris pH 5.5, 0.1M Ammonium Acetate