8YPA

Human mitochondrial ClpP in complex with TR89

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	142.882, 152.424, 104.446
Unit cell angles	90.00, 118.05, 90.00

Refinement procedure

Resolution	46.090 - 2.670
R-factor	0.2161
Rwork	0.215
R-free	0.25840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.055
Data reduction software	XDS
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.090	2.765
High resolution limit [Å]	2.670	2.670
Rmerge	0.200
Rmeas	0.216
Rpim	0.082
Number of reflections	55979	5582
<I/σ(I)>	8.52
Completeness [%]	99.9
Redundancy	6.8
CC(1/2)	0.992	0.755

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	289.15	0.1 M sodium acetate ph 6.2, 8% PEG 4000