8YP7
Crystal structure of glucosyltransferase RrUGT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.191, 70.826, 92.662 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.370 - 1.870 |
| R-factor | 0.18112 |
| Rwork | 0.179 |
| R-free | 0.21208 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.624 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.370 | 1.910 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.090 | 2.018 |
| Rmeas | 0.093 | 2.099 |
| Rpim | 0.026 | 0.572 |
| Total number of observations | 27957 | |
| Number of reflections | 38051 | 2124 |
| <I/σ(I)> | 20.6 | 1.4 |
| Completeness [%] | 99.2 | |
| Redundancy | 13.1 | 13.2 |
| CC(1/2) | 1.000 | 0.585 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.05 M Citric acid, 0.05 M BIS-TRIS propane, pH 4.5, 19% PEG3350 |
