8YO8

Crystal structure of hFSP1 with both 6-OH-FAD and NAD (hFSP1-6-OH-FAD-NAD)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	P 32 2 1
Unit cell lengths	60.162, 60.162, 195.256
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.540 - 2.000
R-factor	0.1877
Rwork	0.186
R-free	0.22250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.878
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.540	2.070
High resolution limit [Å]	2.000	2.000
Rpim	0.030	0.425
Number of reflections	28766	2813
<I/σ(I)>	17.2
Completeness [%]	84.0
Redundancy	19.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.7	291.15	0.1 M Sodium chloride, 0.12 M Lithium sulfate, 0.1 M Sodium HEPES, 16.5% PEG 6000