8YNR

Crystal Structure of Human Rab23 in complex with GMPPNP (1.8 Angstroms Resolution)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-X
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-16
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.5418
Spacegroup name	P 2 21 21
Unit cell lengths	38.677, 61.333, 70.601
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.980 - 1.800
R-factor	0.2048
Rwork	0.203
R-free	0.23120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.030
Data reduction software	CrysalisPro
Data scaling software	CrysalisPro
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	14.980	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.109	1.578
Rmeas	0.114	1.655
Rpim	0.033	0.494
Total number of observations	182062	10465
Number of reflections	16159	960
<I/σ(I)>	19.2	1.5
Completeness [%]	99.8
Redundancy	11.3	10.9
CC(1/2)	0.999	0.956

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	0.1M SPG buffer pH 5.0, 20% PEG 1500