8YN0
Crystal structure of NRG1C in complex with EDS1-SAG101-(ADPr-ATP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.521, 154.914, 171.110 |
| Unit cell angles | 90.00, 89.69, 90.00 |
Refinement procedure
| Resolution | 39.770 - 2.490 |
| R-factor | 0.1991 |
| Rwork | 0.199 |
| R-free | 0.23790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.449 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmeas | 0.150 | 0.990 |
| Number of reflections | 159460 | 7943 |
| <I/σ(I)> | 9.1 | 1.3 |
| Completeness [%] | 96.1 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 5% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.1 M HEPES pH 7.5, 10 % w/v Polyethylene glycol 10000 |
