8YM3

Structural basis of Elongation factor Tu in regulating photoinhibition in Synechococcus sp. PCC 7942.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9980
Spacegroup name	P 1 21 1
Unit cell lengths	54.897, 86.548, 60.775
Unit cell angles	90.00, 95.74, 90.00

Refinement procedure

Resolution	42.710 - 2.050
R-factor	0.1869
Rwork	0.185
R-free	0.22240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.458
Data reduction software	HKL-3000 (7.21)
Data scaling software	HKL-3000 (7.21)
Phasing software	PHASER (2.70)
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.710	2.100
High resolution limit [Å]	2.050	2.050
Rmerge	0.200	1.758
Rmeas	0.403	1.919
Rpim	0.160	0.756
Total number of observations		17421
Number of reflections	35495	2637
<I/σ(I)>	5	2.1
Completeness [%]	100.0
Redundancy	6.5	6.6
CC(1/2)	0.909	0.283

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	289	0.085 M HEPES sodium pH 7.5, 1.7% v/v Polyethylene glycol 400, 1.7 M Ammonium sulfate, 15% v/v Glycerol