8YLZ

Structure of a cis-Geranylfarnesyl Diphosphate Synthase from Streptomyces clavuligerus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97880
Spacegroup name	P 21 2 21
Unit cell lengths	42.580, 61.140, 84.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.570 - 1.600
R-factor	0.1969
Rwork	0.195
R-free	0.22640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.917
Data reduction software	iMOSFLM
Data scaling software	pointless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.940	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.045	0.127
Rmeas	0.048	0.135
Rpim	0.015	0.044
Total number of observations	298879	12444
Number of reflections	27160	1362
<I/σ(I)>	33.2	11.9
Completeness [%]	91.3
Redundancy	11	9.1
CC(1/2)	0.998	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.7	289.15	18% (w/v) Polyethylene glycol 4000, 0.1 M MES monohydrate (pH 5.7)