8YLY
Structural basis of Elongation factor Tu in regulating photoinhibition in Synechococcus sp. PCC 7942.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.244, 86.938, 61.255 |
| Unit cell angles | 90.00, 95.87, 90.00 |
Refinement procedure
| Resolution | 30.470 - 1.490 |
| R-factor | 0.1771 |
| Rwork | 0.176 |
| R-free | 0.19270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.623 |
| Data reduction software | HKL-3000 (7.21) |
| Data scaling software | HKL-3000 (7.21) |
| Phasing software | PHASER (2.7.0) |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.930 | 1.570 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.046 | 0.442 |
| Rmeas | 0.052 | 0.525 |
| Rpim | 0.024 | 0.275 |
| Total number of observations | 403107 | 47566 |
| Number of reflections | 90042 | 13636 |
| <I/σ(I)> | 15.7 | 2.7 |
| Completeness [%] | 95.4 | |
| Redundancy | 4.5 | 3.5 |
| CC(1/2) | 0.999 | 0.786 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 289 | 0.085 M HEPES sodium pH 7.5, 1.7% v/v Polyethylene glycol 400, 1.7 M Ammonium sulfate, 15% v/v Glycerol |
