8YLG

Crystal structure of Burkholderia thailandensis MftR in complex with operator DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2021-03-31
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	62.554, 101.580, 109.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.890 - 1.800
R-factor	0.1978
Rwork	0.197
R-free	0.21980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	in-house Se-SAD model
RMSD bond length	0.007
RMSD bond angle	0.956
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.830
High resolution limit [Å]	1.800	1.800
Number of reflections	65149	3166
<I/σ(I)>	23.2	1.8
Completeness [%]	99.6	99
Redundancy	4.9	4.9
CC(1/2)	0.997	0.687

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	PEG 8000, sodium cacodylate, calcium acetate