8YLA
Crystal structures of terpene synthases complexed with a substrate mimic
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97902 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.308, 83.623, 80.055 |
| Unit cell angles | 90.00, 122.80, 90.00 |
Refinement procedure
| Resolution | 28.650 - 1.300 |
| R-factor | 0.1762 |
| Rwork | 0.175 |
| R-free | 0.19140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.027 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.106 | 0.493 |
| Number of reflections | 149763 | 14701 |
| <I/σ(I)> | 21.5 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 200 mM Magnesium formate and 20% w/v PEG3350 |
