8YL2

Crystal Structure of Homo Tetrameric RagA-like small GTPase from Asgard Lokiarchaeota (LokiRagO) in complex with GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-23
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.000032
Spacegroup name	P 1 21 1
Unit cell lengths	118.756, 97.183, 145.738
Unit cell angles	90.00, 104.28, 90.00

Refinement procedure

Resolution	27.250 - 2.200
R-factor	0.2035
Rwork	0.202
R-free	0.22950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.519
Data reduction software	DIALS (3.18.0)
Data scaling software	DIALS (3.18.0)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.250	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.102	1.666
Rpim	0.041	0.750
Number of reflections	162816	7967
<I/σ(I)>	12.9	0.8
Completeness [%]	99.9	99.6
Redundancy	7	5.9
CC(1/2)	0.998	0.385

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	292.15	Poly Ethylene Glycol (PEG) 6,000 and 2-Methyl-2,4-pentanediol (MPD).