8YKT
Fab and SEB complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-26 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.250, 73.320, 114.220 |
| Unit cell angles | 90.00, 91.66, 90.00 |
Refinement procedure
| Resolution | 38.060 - 2.040 |
| R-factor | 0.1972 |
| Rwork | 0.195 |
| R-free | 0.22970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.572 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.330 | 2.090 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.155 | 1.405 |
| Rmeas | 0.168 | 1.519 |
| Rpim | 0.065 | 0.573 |
| Total number of observations | 380788 | 28725 |
| Number of reflections | 55954 | 4118 |
| <I/σ(I)> | 10.4 | 1.7 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.8 | 7 |
| CC(1/2) | 0.997 | 0.552 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M Lithum sulfate, 0.1M Sodium acetate pH5.5, 10%PEG1000, 10% PEG8000 |
