8YK1
Blood group B alpha-1,3-galactosidase AgaBb from Bifidobacterium bifidum, construct 23-844
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2022-02-27 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.0520 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 127.689, 68.771, 291.306 |
Unit cell angles | 90.00, 91.40, 90.00 |
Refinement procedure
Resolution | 48.590 - 2.020 |
R-factor | 0.1907 |
Rwork | 0.189 |
R-free | 0.22130 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.824 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.590 | 2.060 |
High resolution limit [Å] | 2.020 | 2.020 |
Number of reflections | 161615 | 5145 |
<I/σ(I)> | 8.9 | 2.7 |
Completeness [%] | 98.2 | |
Redundancy | 7.1 | |
CC(1/2) | 1.000 | 0.880 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 10% (w/v) PEG 3350, 0.1M sodium iodide (seed condition: 10% (w/v) PEG 6000, 0.1M citric acid pH 5.0) |