8YJN

Structure of E. coli glycyl radical enzyme YbiW with bound glycerol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-03-28
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.95370
Spacegroup name	F 2 2 2
Unit cell lengths	114.926, 207.040, 210.555
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.840 - 2.420
R-factor	0.1756
Rwork	0.174
R-free	0.21290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.868
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.950	2.510
High resolution limit [Å]	2.420	2.420
Rmerge	0.067	0.495
Rmeas	0.072	0.531
Rpim	0.026	0.193
Total number of observations	358154	34768
Number of reflections	47693	4632
<I/σ(I)>	16.9	3.3
Completeness [%]	99.9
Redundancy	7.5	7.5
CC(1/2)	0.999	0.945

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.1M Imidazole pH 8.0, 1 M sodium acetate