8YJ8
Characerization of a novel format scFvXVHH single-chain Biparatopic antibody against a metal binding protein, MtsA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-11-22 |
| Detector | RAYONIX MX225-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 292.931, 58.083, 50.760 |
| Unit cell angles | 90.00, 91.24, 90.00 |
Refinement procedure
| Resolution | 35.570 - 1.650 |
| R-factor | 0.1895 |
| Rwork | 0.188 |
| R-free | 0.22770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hh8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.865 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.270 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.070 | 1.116 |
| Rpim | 0.043 | 0.670 |
| Number of reflections | 101614 | 3190 |
| <I/σ(I)> | 8.4 | 1.1 |
| Completeness [%] | 99.3 | 98.05 |
| Redundancy | 3.72 | 3.69 |
| CC(1/2) | 0.997 | 0.569 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.2 M Ammonium sulfate, 20% w/v PEG 3,350 |
