8YJ7
Characerization of a novel format scFvXVHH single-chain Biparatopic antibody against a metal binding protein, MtsA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-11-01 |
Detector | RAYONIX MX225-HS |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.591, 129.272, 245.588 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.270 - 2.800 |
R-factor | 0.2243 |
Rwork | 0.222 |
R-free | 0.27600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hh8 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.746 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.530 | 2.920 |
High resolution limit [Å] | 2.800 | 2.820 |
Rmerge | 0.209 | 1.178 |
Rpim | 0.082 | |
Number of reflections | 57771 | 2603 |
<I/σ(I)> | 10 | |
Completeness [%] | 99.9 | |
Redundancy | 7.38 | |
CC(1/2) | 0.994 | 0.637 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.05 M Zinc acetate dihydrate, 20% w/v PEG 3350 |