8YJ5

Characerization of a novel format scFvXVHH single-chain Biparatopic antibody against a metal binding protein, MtsA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2021-11-01
Detector	RAYONIX MX225-HS
Wavelength(s)	1.0
Spacegroup name	I 41
Unit cell lengths	120.450, 120.450, 156.176
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.790 - 3.660
R-factor	0.2651
Rwork	0.263
R-free	0.30330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hh8
RMSD bond length	0.023
RMSD bond angle	2.037
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.790	3.790
High resolution limit [Å]	3.660	3.660
Rmerge	0.177	1.891
Rpim	0.069	0.746
Number of reflections	12349	3077
<I/σ(I)>	7.9	1
Completeness [%]	99.9
Redundancy	7.61
CC(1/2)	0.996	0.452

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	1.8 M Ammonium citrate tribasic