8YJ2

N17.1.2 recognition of NRAS neoantigens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	C 2 2 21
Unit cell lengths	71.524, 134.158, 222.128
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.776 - 2.261
R-factor	0.1909
Rwork	0.189
R-free	0.22860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.929
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.800	2.340
High resolution limit [Å]	2.260	2.260
Number of reflections	464104
<I/σ(I)>	13.5
Completeness [%]	98.5
Redundancy	9.3
CC(1/2)	0.985	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10mg/ml, 0.2 M NH4 citrate tribasic, 16% PEG3350, pH 7.4