8YJ2
N17.1.2 recognition of NRAS neoantigens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9793 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 71.524, 134.158, 222.128 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.776 - 2.261 |
R-factor | 0.1909 |
Rwork | 0.189 |
R-free | 0.22860 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.929 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.16_3549: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.800 | 2.340 |
High resolution limit [Å] | 2.260 | 2.260 |
Number of reflections | 464104 | |
<I/σ(I)> | 13.5 | |
Completeness [%] | 98.5 | |
Redundancy | 9.3 | |
CC(1/2) | 0.985 | 0.883 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10mg/ml, 0.2 M NH4 citrate tribasic, 16% PEG3350, pH 7.4 |