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8YJ2

N17.1.2 recognition of NRAS neoantigens

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 23-ID-B
Synchrotron siteAPS
Beamline23-ID-B
Temperature [K]100
Detector technologyPIXEL
Collection date2022-02-14
DetectorDECTRIS EIGER X 16M
Wavelength(s)0.9793
Spacegroup nameC 2 2 21
Unit cell lengths71.524, 134.158, 222.128
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution42.776 - 2.261
R-factor0.1909
Rwork0.189
R-free0.22860
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.008
RMSD bond angle0.929
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHENIX
Refinement softwarePHENIX ((1.16_3549: ???))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]42.8002.340
High resolution limit [Å]2.2602.260
Number of reflections464104
<I/σ(I)>13.5
Completeness [%]98.5
Redundancy9.3
CC(1/2)0.9850.883
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP29310mg/ml, 0.2 M NH4 citrate tribasic, 16% PEG3350, pH 7.4

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