8YIK
pP1192R-ATPase-domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-31 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 85.753, 85.753, 211.483 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.070 - 1.300 |
| R-factor | 0.2004 |
| Rwork | 0.200 |
| R-free | 0.20630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.902 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 211.480 | 1.330 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.092 | |
| Number of reflections | 111723 | 1531 |
| <I/σ(I)> | 0 | |
| Completeness [%] | 98.7 | |
| Redundancy | 5 | |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20%(W/V) PEG 3350 |
