8YIA

The selenomethionine contained structure of SdnG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97843
Spacegroup name	P 21 21 21
Unit cell lengths	73.605, 73.611, 108.773
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.940 - 1.430
R-factor	0.1955
Rwork	0.194
R-free	0.21870
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.022
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.450
High resolution limit [Å]	1.430	1.430
Rmerge	0.068	1.519
Number of reflections	109071	109071
<I/σ(I)>	41.214	4.2
Completeness [%]	99.2
Redundancy	13.1
CC(1/2)		0.662

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289.15	0.2M 1,6-hexanediol, 0.2M 1-butanol, 0.2M (RS)-1,2- propanediol, 0.2M 2-propanol, 0.2M 1,4-butanediol, 0.2M 1,3-propanediol, 0.1M Bicine/Tris pH 8.5