8YHP
Structure of the PGK1 from Biortus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-24 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.18075 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 61.591, 70.058, 95.681 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.300 - 1.950 |
Rwork | 0.234 |
R-free | 0.28640 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.898 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.840 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.094 | 0.742 |
Number of reflections | 30843 | 2162 |
<I/σ(I)> | 12.9 | |
Completeness [%] | 99.8 | |
Redundancy | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Na/K PO4, 20% PEG 3,350 |