8YHK

The Crystal Structure of P21-Activated Kinases Pak4 from Biortus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.95373
Spacegroup name	P 43 21 2
Unit cell lengths	146.274, 146.274, 43.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.299 - 3.300
Rwork	0.249
R-free	0.31860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	1.195
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.760	3.560
High resolution limit [Å]	3.300	3.300
Number of reflections	7176	1460
<I/σ(I)>	9.6
Completeness [%]	95.1
Redundancy	11
CC(1/2)	0.996	0.809

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.4 M Potassium sodium tartrate tetrahydrate