8YHK
The Crystal Structure of P21-Activated Kinases Pak4 from Biortus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-02-10 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.95373 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 146.274, 146.274, 43.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.299 - 3.300 |
Rwork | 0.249 |
R-free | 0.31860 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 1.195 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.760 | 3.560 |
High resolution limit [Å] | 3.300 | 3.300 |
Number of reflections | 7176 | 1460 |
<I/σ(I)> | 9.6 | |
Completeness [%] | 95.1 | |
Redundancy | 11 | |
CC(1/2) | 0.996 | 0.809 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.4 M Potassium sodium tartrate tetrahydrate |