8YFL

crystal structure of FIP200 claw/TNIP1_FIR_pS122pS123

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 32
Unit cell lengths	60.923, 60.923, 65.394
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.380 - 1.500
R-factor	0.2011
Rwork	0.200
R-free	0.21770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.722
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.380	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.063	0.318
Number of reflections	43384	2076
<I/σ(I)>	19.6	3.7
Completeness [%]	99.8
Redundancy	9.7
CC(1/2)	0.998	0.966

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	15% w/v PEG 6000, 0.1M MES, 5% v/v MPD, pH 6.5