8YFJ

TRIP4 ASCH domain in complex with a 12bp dsDNA (5'-ATTGGATCCAAT-3')

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 61 2 2
Unit cell lengths	67.496, 67.496, 198.082
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.780 - 1.840
R-factor	0.2105
Rwork	0.210
R-free	0.22790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.917
Data scaling software	Aimless
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.460	1.890
High resolution limit [Å]	1.840	1.840
Rmerge	0.030	1.118
Rmeas	0.313	1.146
Rpim	0.052	0.247
Total number of observations	824803	35900
Number of reflections	24210	1735
<I/σ(I)>	9.3	3
Completeness [%]	100.0
Redundancy	34.1	20.7
CC(1/2)	0.986	0.538

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	2.0 M (NH4)2SO4