8YFI

TRIP4 ASCH domain in complex with a 12bp dsDNA (5'-TGAGGTACCTCA-3')

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 61 2 2
Unit cell lengths	66.263, 66.263, 199.250
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.131 - 2.020
R-factor	0.2061
Rwork	0.204
R-free	0.24880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.977
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.050
High resolution limit [Å]	2.020	5.480	2.020
Rmerge	0.062	0.025	0.710
Rmeas	0.068	0.028	0.773
Rpim	0.027	0.012	0.301
Total number of observations	109981
Number of reflections	17897	1031	866
<I/σ(I)>	4.3
Completeness [%]	99.7	96.5	99.8
Redundancy	6.1	5.2	6.4
CC(1/2)	0.999	0.999	0.858

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	2.0 M (NH4)2SO4