8YFH
Structure of alpha-1,3-glucanase agn1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-24 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.534, 114.514, 109.111 |
| Unit cell angles | 90.00, 104.24, 90.00 |
Refinement procedure
| Resolution | 19.990 - 1.800 |
| R-factor | 0.1667 |
| Rwork | 0.165 |
| R-free | 0.19140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.776 |
| Data scaling software | XDS |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.010 | 1.810 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Number of reflections | 158150 | 7813 |
| <I/σ(I)> | 21.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 5.2 | |
| CC(1/2) | 0.999 | 0.791 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.9M Disodium Malonate, 0.1M HEPES pH 7.0 |
