8YER

Tubulin-RB3_SLD-TTL in complex with compound 4

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	105.150, 157.260, 181.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.700 - 2.710
R-factor	0.2239
Rwork	0.222
R-free	0.28590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond angle	12.566
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20_4459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.700	2.780
High resolution limit [Å]	2.710	2.710
Number of reflections	82432	6022
<I/σ(I)>	0.257
Completeness [%]	100.0
Redundancy	7.5
CC(1/2)	0.980	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	6% PEG 4000, 5% Glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2