8YEA

Room temperature structure of TsaGH11 determined by macromolecular crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	299
Detector technology	PIXEL
Collection date	2019-12-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9794
Spacegroup name	P 43 21 2
Unit cell lengths	69.896, 69.896, 170.428
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.470 - 2.850
R-factor	0.1905
Rwork	0.183
R-free	0.25620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.920
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.850	2.850
Number of reflections	10422	459
<I/σ(I)>	9.2
Completeness [%]	94.4
Redundancy	6
CC(1/2)	1.000	0.782

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	295	sodium acetate, ammonium acetate