8YDF

E.coli transcription translation coupling complex in TTC-B state 1 (subclass 2) containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin

This is a non-PDB format compatible entry.

EM specimen

Vitrification

Yes

EM Data Collection

Microscope	FEI TALOS ARCTICA
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	200
Detector	FEI FALCON III (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	4.6
CTF correction	NONE