8YDC

Crystal structure of a hammerhead ribozyme with pseudoknot

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2021-07-04
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	P 61 2 2
Unit cell lengths	83.578, 83.578, 258.790
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	16.250 - 2.890
R-factor	0.196
Rwork	0.195
R-free	0.20910
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.866
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.250	2.993
High resolution limit [Å]	2.890	2.890
Number of reflections	12665	1226
<I/σ(I)>	18.05
Completeness [%]	98.4
Redundancy	37.8
CC(1/2)	1.000	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	Magnesium acetate tetrahydrate MES monohydrate Ammonium sulfate