8YBC

Crystal structure of coiled coil domain of Golm1 (Golgi membrane protein 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	58.810, 44.020, 63.800
Unit cell angles	90.00, 112.92, 90.00

Refinement procedure

Resolution	35.230 - 2.280
R-factor	0.25
Rwork	0.242
R-free	0.32100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.878
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	Arcimboldo
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.230	2.340
High resolution limit [Å]	2.280	2.280
Rmerge	0.126	0.666
Number of reflections	23659	12613
<I/σ(I)>	8	2.2
Completeness [%]	90.5	93.8
Redundancy	3.5
CC(1/2)	0.992	0.759

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	289.15	5% PEG6000