8YA6
endo-1,3-fucanase Fun168A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 53.703, 67.220, 239.443 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.610 - 1.920 |
| R-factor | 0.2163 |
| Rwork | 0.215 |
| R-free | 0.23010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.028 |
| RMSD bond angle | 1.857 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.960 | 1.970 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.090 | 0.153 |
| Rmeas | 0.103 | 0.176 |
| Rpim | 0.048 | 0.085 |
| Total number of observations | 119400 | 7650 |
| Number of reflections | 32524 | 2137 |
| <I/σ(I)> | 11.1 | 6.5 |
| Completeness [%] | 97.1 | |
| Redundancy | 3.7 | 3.6 |
| CC(1/2) | 0.991 | 0.962 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.2 M Sodium acetate trihydrate+0.1 M Sodium cacodylate trihydrate pH 6.5+24% w/v PEG 8000 |
