8YA4

Crystal structure of a lysozyme form hen egg white

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17UM
Synchrotron site	SSRF
Beamline	BL17UM
Temperature [K]	300
Detector technology	PIXEL
Collection date	2023-12-17
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.924
Spacegroup name	P 43 21 2
Unit cell lengths	78.819, 78.819, 37.699
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.410 - 2.100
R-factor	0.1939
Rwork	0.189
R-free	0.23960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.965
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.410	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.291	1.236
Rmeas	0.297	1.262
Rpim	0.059	0.252
Total number of observations	183398	14482
Number of reflections	7407	585
<I/σ(I)>	20.2	6.6
Completeness [%]	100.0
Redundancy	24.8	24.8
CC(1/2)	0.998	0.929

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	300	0.1 M sodium acetate, 6% sodium chloride