8YA1
HEN EGG WHITE LYSOZYME
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17UM |
| Synchrotron site | SSRF |
| Beamline | BL17UM |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-20 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.924 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.258, 78.258, 36.808 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.130 - 1.570 |
| R-factor | 0.1947 |
| Rwork | 0.193 |
| R-free | 0.21330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.114 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.130 | 1.590 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.056 | 0.639 |
| Rmeas | 0.057 | 0.658 |
| Rpim | 0.013 | 0.152 |
| Total number of observations | 311955 | 14320 |
| Number of reflections | 16608 | 788 |
| <I/σ(I)> | 30.9 | 5.2 |
| Completeness [%] | 99.9 | |
| Redundancy | 18.8 | 18.2 |
| CC(1/2) | 1.000 | 0.940 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 300 | 0.1 M sodium acetate, 6% sodium chloride |
