8Y9U
Crystal structure of nanobody MY6323 bound to human serum albumin (HSA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-01-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.420, 65.892, 118.713 |
| Unit cell angles | 104.85, 103.41, 91.73 |
Refinement procedure
| Resolution | 63.400 - 3.100 |
| Rwork | 0.273 |
| R-free | 0.31620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS (0.7.7) |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.400 | 3.260 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.078 | 0.479 |
| Rmeas | 0.093 | 0.568 |
| Rpim | 0.050 | 0.301 |
| Total number of observations | 100447 | 15313 |
| Number of reflections | 29492 | 4404 |
| <I/σ(I)> | 9.6 | 2.6 |
| Completeness [%] | 96.1 | |
| Redundancy | 3.4 | 3.5 |
| CC(1/2) | 0.996 | 0.825 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M Ammonium sulfate, 0.1 M Tris pH 8.5, 23% w/v Polyethylene glycol 3,350 |
